Anti-PFKP Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q01813

Core Product Specifications and Parameters

Biological Background: PFKP Function and Localization

• ATP-dependent 6-phosphofructokinase, platelet type (PFKP) is also known as 6-phosphofructokinase type C, phosphofructo-1-kinase isozyme C (PFK-C), or phosphohexokinase.
• PFKP catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
• This enzyme is an allosteric enzyme, regulated by key metabolites, and plays a critical role in controlling glycolytic flux.
• Subcellular localization is primarily cytoplasmic, consistent with its function in energy metabolism.
• PFKP belongs to the transferase family and requires magnesium as a cofactor for its kinase activity.
• Post-translational modifications include acetylation, glycosylation, and phosphorylation, which may modulate enzyme activity or stability.
• Alternative splicing produces multiple isoforms of PFKP, which may have tissue-specific functions or regulatory properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 336-540 of human PFKP; this region is conserved across the expected cross-reactive species, but alignment verification is recommended for non-human samples.
• For Western blotting, a band at approximately 86 kDa is expected. Validate band specificity using appropriate positive and negative controls.
• For IHC-P, consider optimizing antigen retrieval conditions and antibody dilution for your specific tissue type.
• ELISA performance may depend on the coating antigen and detection format; pilot experiments are advised to establish optimal conditions.
• Cross-reactivity with mouse and rat is supported by supplier data, but independent validation in your model system is recommended.

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CamelBio is your reliable partner for IVD raw material sourcing, offering a comprehensive portfolio including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and access to rare targets like PFKP for glycolysis and cancer metabolism research. From concept to clinic, our expert technical services and consulting streamline your diagnostic development pipelines, ensuring quality and efficiency. Let CamelBio be your strategic sourcing bridge to accelerate your next project.