Anti-PFKM Rabbit Monoclonal Antibody for WB - P08237

Core Product Specifications and Parameters

Biological Background: PFKM Function and Localization

• PFKM encodes the muscle-specific isoform of ATP-dependent 6-phosphofructokinase, also known as phosphofructo-1-kinase isozyme A (PFK-A) or phosphohexokinase.
• It catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate, the first committing step of glycolysis, using ATP as a phosphate donor.
• This enzyme is an allosteric regulator of glycolysis, responding to cellular energy status, and is critical for muscle ATP production.
• Mutations in PFKM cause glycogen storage disease type 7 (Tarui disease), an autosomal recessive disorder characterized by exercise-induced muscle cramps and myoglobinuria (disease variant).
• PFKM localizes to the cytoplasm and undergoes multiple post-translational modifications, including phosphorylation, acetylation, hydroxylation, and glycosylation.
• It requires magnesium for catalytic activity and possesses a nucleotide-binding domain for ATP (metal-binding, ATP-binding).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 50–150 of human PFKM; consider epitope mapping to confirm binding specificity.
• For Western blot, the predicted molecular weight is approximately 85 kDa. Use appropriate reducing conditions and validate antibody dilution in your sample system.
• The antibody has been tested for ELISA; for sandwich applications, pairing with a detection antibody may require optimization.
• Cross-reactivity with mouse and rat makes this antibody suitable for translational and comparative studies; always verify cross-reactivity in your experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to a comprehensive portfolio of IVD raw materials, technical services, and consulting. This PFKM antibody supports glycolysis and metabolic disease research, and CamelBio can facilitate procurement of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk reagents, and rare target sourcing to accelerate your assay development from concept to clinic.