Anti-PFKL Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P17858

Core Product Specifications and Parameters

Biological Background: PFKL Function and Localization

• PFKL (ATP-dependent 6-phosphofructokinase, liver type), also known as PFK-B, PFK-L, or phosphohexokinase, is a ~85 kDa glycolytic enzyme belonging to the phosphofructokinase family.
• Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed:22923583). Related references: PMID:22923583
• Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis; upon macrophage activation, it drives the metabolic switch toward glycolysis, reducing glucose turnover via the pentose phosphate pathway.
• Localizes primarily in the cytoplasm, consistent with its role in glycolysis.
• Functions as an allosteric enzyme regulated by ATP and other metabolites; undergoes post-translational modifications including acetylation and phosphorylation.
• Alternative splicing produces multiple isoforms, and the protein has been identified through direct protein sequencing and proteomics analyses.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to the C-terminal region (aa 680–780) of human PFKL, a conserved sequence that likely underlies the reported cross-reactivity with mouse and rat.
• For Western blotting, a major band near 85 kDa is expected; consider optimizing sample lysis and electrophoresis conditions to achieve clear separation.
• In IHC-P, using appropriate antigen retrieval methods and validating with known positive and negative tissue controls may improve staining specificity.
• For ELISA, the antibody can be tested as either capture or detection reagent; empirical testing is recommended to establish optimal pairings and concentrations.
• As a rabbit monoclonal antibody, it provides consistent lot-to-lot performance, which is advantageous for developing reproducible immunoassays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing platform for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our portfolio includes critical targets like PFKL, essential for metabolism, cancer, and immunology research. Whether you need rare target raw-material sourcing or full development support from concept to clinic, CamelBio is your partner in accelerating assay development.