Anti-PFKFB3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q16875

Core Product Specifications and Parameters

Biological Background: PFKFB3 Function and Localization

• PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3) is a bifunctional enzyme that catalyzes both the synthesis and degradation of fructose-2,6-bisphosphate, a key regulator of glycolysis.
• It belongs to the PFKFB family of enzymes and is also known as iPFK-2 (inducible PFK-2) and renal carcinoma antigen NY-REN-56.
• The protein is expressed ubiquitously across human tissues.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.
• PFKFB3 is a phosphoprotein, indicating regulation by phosphorylation events.
• The canonical isoform has a calculated molecular weight of approximately 59.6 kDa (UniProt), consistent with the commonly observed ~60 kDa band in western blotting.
• As a kinase/hydrolase, PFKFB3 contains both ATP-binding and nucleotide-binding domains essential for its enzymatic activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-210) of human PFKFB3, suggesting that the antibody may recognize multiple isoforms containing this sequence.
• For western blotting, a primary band around 60 kDa is expected; consider optimizing blocking and detection conditions for your specific sample lysates.
• In IF/ICC, appropriate fixation and permeabilization methods should be empirically determined to preserve antigenicity and accessibility.
• The antibody cross-reacts with mouse PFKFB3, making it suitable for studies in murine models; validate performance in the relevant species and tissue context.
• For ELISA applications, pairing with a suitable capture/detection antibody and validating matrix effects are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers with comprehensive raw-material solutions, from concept to clinic. This anti-PFKFB3 antibody is part of our extensive portfolio of validated research and IVD antibodies, and we offer custom bulk production, antibody development, and ancillary reagent sourcing to streamline your assay development. Whether you need optimized antibody pairs, monoclonal alternatives, or rare target raw materials, CamelBio is your trusted partner in metabolic and oncology research.