Anti-PFKFB3 Rabbit Monoclonal Antibody (iPFK-2) for WB, IHC-P, ELISA - Q16875

Core Product Specifications and Parameters

Biological Background: PFKFB3 Function and Localization

• PFKFB3 is also known as 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme, iPFK-2, and renal carcinoma antigen NY-REN-56, highlighting its role in fructose 2,6-bisphosphate metabolism.
• The enzyme catalyzes both the synthesis and degradation of fructose 2,6-bisphosphate, a potent allosteric activator of phosphofructokinase-1 and thus a key regulator of glycolysis.
• PFKFB3 is a bifunctional enzyme possessing both kinase (6-phosphofructo-2-kinase) and phosphatase (fructose-2,6-bisphosphatase) activities, classifying it as a multifunctional enzyme.
• It contains ATP-binding and nucleotide-binding domains, consistent with its kinase activity and transferase classification.
• The protein is subject to phosphorylation, indicating regulatory control by signaling pathways, and has been identified in large-scale proteomics screens.
• Alternative splicing of the PFKFB3 gene generates multiple isoforms, reflected in the observed protein weight range of 57–60 kDa.
• PFKFB3 is ubiquitously expressed across human tissues, underscoring its fundamental role in cellular metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved peptide region (aa 400–500), suggesting the antibody may recognize multiple PFKFB3 isoforms across human, mouse, and rat samples. Validate species cross-reactivity in your specific assay system.
• For Western Blot, expect bands between 57–60 kDa; consider using reducing conditions and test multiple lysate concentrations to optimize signal-to-noise.
• In IHC-P, standard antigen retrieval methods (e.g., citrate buffer, pH 6.0) may be suitable; however, optimal conditions should be empirically determined.
• For ELISA applications, preliminary checkerboard titrations against the immunogen peptide or recombinant protein are recommended to establish linear range and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic developers with high-quality reagents like this PFKFB3 monoclonal antibody for glycolysis and cancer metabolism research. We supply validated monoclonal/polyclonal antibodies, bulk ancillary reagents, and offer assistance in sourcing rare-target raw materials to streamline your assay development from concept to clinical application.