Anti-PFDN5 Rabbit Monoclonal Antibody for WB, IF, ELISA - Q99471

Core Product Specifications and Parameters

Biological Background: Prefoldin subunit 5 Function and Localization

• Prefoldin subunit 5 (PFDN5, also known as MM-1) is a component of the prefoldin chaperone complex that binds nascent polypeptide chains and transfers them to the cytosolic chaperonin for proper folding.
• PFDN5 specifically interacts with c-MYC and represses its transcriptional activity, thereby functioning as a modulator of MYC-mediated cell proliferation.
• The protein localizes to both the nucleus and cytoplasm, indicating shuttling between these compartments, which is consistent with its roles in protein folding and transcriptional regulation.
• Expression is highest in the pancreas and skeletal muscle, with moderate levels in other tissues, suggesting tissue-specific regulatory functions.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its activity and interactions.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity.
• As a chaperone, PFDN5 helps maintain protein homeostasis by preventing aggregation in the crowded cellular environment.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic peptide corresponding to amino acids 50–154 of human PFDN5. For Western blot (WB), consider using a standard SDS-PAGE and transfer protocol, as the predicted molecular weight is approximately 17 kDa.
• For immunofluorescence (IF) applications, optimize fixation and permeabilization conditions, as PFDN5 localizes to both the nucleus and cytoplasm.
• When using ELISA, validate the antibody’s binding to the immunogen peptide or recombinant protein under the specific assay conditions.
• Given the reported cross-reactivity with mouse and rat, the antibody may be suitable for these species, but validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines diagnostic and research procurement by offering high-quality IVD raw materials, including this PFDN5 monoclonal antibody, to support prefoldin and MYC modulation studies. Our one-stop platform provides validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, enabling seamless transition from concept to clinical application.