Anti-PFDN2 Polyclonal Antibody for WB, ELISA - Q9UHV9

Core Product Specifications and Parameters

Biological Background: Prefoldin subunit 2 Function and Localization

• Prefoldin subunit 2 (PFDN2), also known as PFD2, is a component of the prefoldin complex, a hexameric molecular chaperone involved in protein folding.
• PFDN2 specifically binds to nascent polypeptide chains and cytosolic chaperonin (c-CPN), facilitating the transfer and proper folding of target proteins in a competitive cellular environment.
• The protein is implicated in general chaperone activities, ensuring correct protein conformation and preventing aggregation.
• Subcellular localization studies indicate presence in the nucleus, cytoplasm, and mitochondrion, suggesting roles in multiple cellular compartments.
• As part of the prefoldin family, PFDN2 contributes to the assembly and stabilization of proteins, particularly those destined for cytoskeletal structures.
• The three-dimensional structure of PFDN2 has been solved, providing insights into its mechanistic function in chaperone-assisted folding.
• PFDN2 is a reference proteome component, highlighting its fundamental biological significance across species.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to the full-length human PFDN2 (amino acids 1-154), which may provide broad epitope coverage. Consider this when designing detection protocols for native PFDN2.
• For Western blot (WB) applications, validate antibody specificity using appropriate positive and negative control lysates (e.g., human and mouse cell lines expressing PFDN2).
• In ELISA, optimize coating antigen and antibody dilution to achieve desired sensitivity, and cross-verify with known standards if available.
• Given the protein's small size (~17 kDa), ensure proper gel percentage and transfer conditions for WB to avoid band migration or transfer issues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-PFDN2 antibody. Whether you need bulk ancillary reagents or rare target sourcing for chaperone and protein folding research, our integrated supply pipeline supports accelerating your assay development from concept to clinic.