Anti-PFDN1 Polyclonal Antibody for WB, ELISA - O60925

Core Product Specifications and Parameters

Biological Background: PFDN1 Function and Localization

• PFDN1, also known as PDF or PFD1, encodes prefoldin subunit 1, a core component of the heterohexameric prefoldin chaperone complex.
• It specifically binds to cytosolic chaperonin (c-CPN) and mediates the transfer of nascent polypeptide chains to the chaperonin for ATP-dependent folding.
• PFDN1 binds directly to newly synthesized polypeptides, preventing misfolding and aggregation in the crowded cytosolic environment.
• Structural studies have provided 3D models of the prefoldin complex, and the protein is subject to post-translational acetylation.
• Identified as part of the reference human proteome, with a molecular weight of approximately 14.2 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-122) of human PFDN1; cross-reactivity with mouse and rat orthologs is indicated, so validate reactivity in your specific model.
• For Western blot, a band at ~14 kDa is expected; use reducing SDS-PAGE and include a positive control lysate (e.g., HeLa).
• In ELISA, this antibody can be employed for capture or detection of recombinant PFDN1; optimize coating concentration and blocking agent empirically.
• Consider testing both reducing and non-reducing conditions to distinguish monomeric PFDN1 from potential prefoldin complexes.

CamelBio: Your One-Stop Sourcing Bridge

This PFDN1 polyclonal antibody exemplifies the quality raw materials CamelBio provides for protein folding and chaperone research. We offer a comprehensive range of validated antibody pairs, bulk monoclonals and polyclonals, and ancillary reagents, along with custom sourcing for hard-to-find targets. Partner with CamelBio for integrated IVD raw material solutions that accelerate your project from concept to clinic.