Anti-PFAS Polyclonal Antibody for WB, ELISA - O15067

Core Product Specifications and Parameters

Biological Background: PFAS Function and Localization

• PFAS (phosphoribosylformylglycinamidine synthase), also known as FGAMS, PURL, FGAR amidotransferase (FGARAT), GATD8, or FGAR-AT, is a key enzyme in the de novo purine biosynthetic pathway.
• It catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to formylglycinamidine ribonucleotide (FGAM) and glutamate, a critical step in the assembly of the purine ring.
• The enzyme belongs to the glutamine amidotransferase family and possesses distinct domains for glutamine amide transfer and ATP-dependent ligase activity.
• PFAS is primarily localized in the cytoplasm, where purine biosynthesis occurs.
• UniProt keywords highlight its roles in ATP binding, nucleotide binding, metal binding (magnesium), and its classification as a ligase and phosphoprotein.
• The full-length human protein has a calculated molecular weight of approximately 144.7 kDa (UniProt), consistent with the observed 145 kDa by Western blot.
• PFAS is part of a conserved metabolic pathway, and its human, mouse, and rat orthologs share high sequence identity, underpinning the broad cross-reactivity of this antibody.

Experimental Guidance and Technical Tips

• For Western blot, consider using a standard SDS-PAGE protocol and expect a band near 145 kDa under reducing conditions; optimize blocking and antibody dilution to minimize background.
• In ELISA applications, the polyclonal nature of the antibody may provide robust signal; titrate both capture and detection conditions for best sensitivity.
• Due to the high sequence conservation among human, mouse, and rat, this antibody is likely suitable for cross-species studies; however, validate performance in your specific sample matrix and species.
• The immunogen (N-terminal residues 1-300) covers a region outside the catalytic core, which may allow recognition of both native and denatured PFAS; test in your relevant cell or tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, core labs, and research institutes with streamlined access to IVD raw materials and technical support—from early development to clinical application. This anti-PFAS polyclonal antibody supports purine metabolism research and immunoassay design, and CamelBio can complement it with validated antibody pairs, bulk reagents, and sourcing of rare-target raw materials. Partner with us to accelerate your diagnostic pipeline with reliable, high-quality immunological tools.