Anti-PEX6 Rabbit pAb for WB, ELISA - Q13608

Core Product Specifications and Parameters

Biological Background: PEX6 Function and Localization

• PEX6 is a peroxisomal biogenesis factor (peroxin-6) that functions as an AAA ATPase, essential for peroxisome protein import.
• It forms the PEX1-PEX6 AAA ATPase complex, a protein dislocase that mediates ATP-dependent extraction of the PEX5 receptor from peroxisomal membranes, enabling PEX5 recycling. Related references: PMID:16314507, PMID:16854980, PMID:21362118
• The complex specifically recognizes PEX5 monoubiquitinated at Cys-11 and pulls it through the PEX2-PEX10-PEX12 retrotranslocation channel, accompanied by unfolding of TPR repeats and cargo release. Related references: PMID:29884772
• PEX6 is encoded by the PEX6 gene, with alternative splicing producing isoforms; mutations are associated with peroxisome biogenesis disorders including Zellweger syndrome and amelogenesis imperfecta.
• Subcellularly, PEX6 localizes to the cytoplasm/cytosol, peroxisome membrane, and cell projections such as the cilium and photoreceptor outer segment.
• It is expressed in the retina, particularly enriched in the photoreceptor layer at the junction between outer and inner segments, where it may support photoreceptor function.
• Post-translational modifications include methylation; the protein contains nucleotide-binding and ATP-binding regions characteristic of AAA ATPases.

Experimental Guidance and Technical Tips

• For Western blotting, a 6–10% SDS-PAGE gel under reducing conditions is recommended to resolve the full-length ~104kDa PEX6; ensure efficient transfer for high-molecular-weight proteins.
• Validate the antibody in lysates from human, mouse, and rat cell lines or tissues, as supported by reported cross-reactivity; confirm specificity in your experimental system.
• In ELISA applications, this polyclonal antibody can be employed as a coating or detection reagent; optimization of antibody pairs may be required for sandwich formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials for peroxisome biology and rare disease applications. We offer a comprehensive portfolio including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for specialized targets like peroxisomal proteins. Our one-stop service streamlines your path from concept to clinic.