Anti-PEX19 Rabbit Monoclonal Antibody for WB and ELISA - P40855

Core Product Specifications and Parameters

Biological Background: PEX19 Function and Localization

• PEX19, also known as peroxin-19, 33 kDa housekeeping protein, or peroxisomal farnesylated protein, is encoded by the PEX19 gene (aliases: PXF, HK33, PMP1, PMPI, PXMP1, PBD12A, D1S2223E) and plays a critical role in early peroxisome biogenesis.
• Functions as a cytosolic chaperone and import receptor for peroxisomal membrane proteins (PMPs): it binds and stabilizes newly synthesized PMPs by interacting with their hydrophobic membrane-spanning domains and targets them to the peroxisomal membrane via the integral membrane protein PEX3.
• Beyond peroxisome biology, PEX19 sequesters CDKN2A in the cytoplasm, preventing its nuclear entry and interaction with MDM2, thereby promoting TP53 degradation and linking peroxisomal function to cell cycle regulation.
• Localizes to both the cytoplasm and the peroxisome membrane, consistent with its dual roles in cytosolic chaperoning and membrane targeting.
• Ubiquitously expressed across human tissues; isoform 1 is strongly predominant in most adult tissues, while isoform 2 is the main form in utero.
• Post-translationally modified by acetylation, phosphorylation, methylation, and prenylation; mutations in PEX19 are associated with peroxisome biogenesis disorders such as Zellweger syndrome.
• The UniProt-calculated molecular weight is 32,807 Da, and alternative splicing produces multiple isoforms with tissue‑specific expression patterns.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to the N-terminal region (aa 1–100) of human PEX19. This region is likely conserved across major isoforms, but isoform‑specific reactivity should be verified if required.
• For western blotting, expect a band near 33 kDa. Ubiquitously expressed cell lines (e.g., HeLa, HEK293) serve as suitable positive controls; consider subcellular fractionation to enrich peroxisomal signal if needed.
• In ELISA, coating conditions may require optimization. For sandwich assays, pairing with a validated detection antibody is recommended; consult the manufacturer for compatible antibody pairs.
• Since PEX19 shuttles between cytoplasm and peroxisome membrane, immunofluorescence co‑staining with a peroxisomal marker (e.g., PMP70) can confirm localization patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and researchers with a comprehensive portfolio of IVD raw materials, including validated monoclonal and polyclonal antibodies, antibody pairs, and bulk ancillary reagents. This anti-PEX19 rabbit monoclonal antibody is tailored for peroxisome biogenesis research and assay development, supporting applications from basic science to clinical diagnostics. For custom conjugations, bulk orders, or rare target sourcing, contact CamelBio to streamline your supply chain from concept to clinic.