Anti-PEX14 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O75381

Core Product Specifications and Parameters

Biological Background: PEX14 Function and Localization

• PEX14 is a peroxisomal membrane protein that functions as a key component of the PEX13-PEX14 docking complex, serving as the initial docking site for the PEX5 receptor-cargo complex during peroxisomal matrix protein import. Related references: PMID:24235149 PMID:28765278 PMID:9653144
• The PEX13-PEX14 translocon can form a dynamic pore that opens to approximately 9 nm, enabling the import of folded cargo proteins.
• PEX14 directly interacts with tubulin, mediating peroxisome movement along microtubules. Related references: PMID:21525035
• It localizes exclusively to the peroxisome membrane, anchoring the docking complex and facilitating protein translocation.
• Mutations in the PEX14 gene cause peroxisome biogenesis disorders, including Zellweger syndrome spectrum.
• Alternative splicing generates multiple PEX14 isoforms, and the protein undergoes post-translational modifications such as acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 118–377 of human PEX14, covering a large portion of the protein; this may provide broad epitope recognition and could detect multiple isoforms.
• In Western blotting, a band near 41 kDa is expected; validate detection in lysates from human or mouse tissues known to express PEX14 (e.g., liver, fibroblasts).
• For immunohistochemistry, peroxisomal membrane localization typically yields punctate cytoplasmic staining; consider titrating the antibody and including appropriate controls.
• In ELISA, the antibody may be used as a capture or detection reagent; pair with a validated PEX14 standard or recombinant fragment.
• Reported cross-reactivity with mouse enables cross-species applications, but sequence alignment should be checked for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research labs with one-stop access to premium IVD raw materials like this anti-PEX14 rabbit polyclonal antibody. From validated antibody pairs and optimized monoclonal/polyclonal reagents to bulk ancillary supplies, our portfolio supports every stage of assay development. Whether you require rare peroxisomal target sourcing or integrated technical solutions, CamelBio streamlines your path from concept to clinic.