Anti-PEX12 Rabbit Polyclonal Antibody for Western Blot & ELISA - O00623

Core Product Specifications and Parameters

Biological Background: PEX12 Function and Localization

• PEX12, also known as Peroxin-12 or PAF-3, is a component of the peroxisomal retrotranslocation channel that, together with PEX2 and PEX10, mediates the export of the PEX5 receptor from the peroxisomal membrane to the cytosol, enabling its recycling (PubMed:24662292, PubMed:9354782, PubMed:9632816). Related references: PMID:24662292 PMID:9354782 PMID:9632816

• PEX12 regulates PEX5 recycling by activating the E3 ubiquitin-protein ligase activity of PEX10; when recycling is compromised, it stimulates PEX10-mediated polyubiquitination of PEX5 for degradation (PubMed:24662292; ubiquitination stimulation by similarity). Related references: PMID:24662292

• Peroxin-12 is an integral membrane protein localized to the peroxisome membrane.

• Mutations in PEX12 are associated with peroxisome biogenesis disorders, including Zellweger syndrome spectrum.

• The protein contains zinc-finger domains and transmembrane helices, indicating roles in metal binding and membrane integration.

• The predicted molecular weight of PEX12 is approximately 40.8 kDa (UniProt), consistent with the product-reported 41 kDa.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the C-terminal region (amino acids 290-359) of human PEX12; this epitope selection may favor detection of the full-length protein.
• For Western blot, validate the antibody in human cell lines that express peroxisomal proteins, such as HepG2 or HeLa, and include appropriate positive and negative controls.
• For ELISA, consider using the recombinant immunogen as a reference antigen to establish standard curves and optimize coating conditions.
• As a polyclonal antibody, lot-to-lot variability is possible; it is advisable to verify each new lot with known standards.
• Cross-reactivity has been confirmed for human; for applications involving other species, experimental validation is recommended.
• Optimize working concentrations experimentally; typical starting dilutions for WB are often in the range of 1:500–1:2000, but this must be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a seamless procurement experience for IVD manufacturers and researchers, providing validated antibody pairs, optimized polyclonal antibodies like this anti-PEX12 product, and bulk ancillary reagents. With expertise spanning early research to clinical applications, CamelBio supports peroxisome biology studies by ensuring access to high-quality raw materials. Whether you need technical consulting or reliable sourcing for rare targets, CamelBio is your dedicated partner from concept to clinic.