Anti-PEX11B Rabbit Polyclonal Antibody for WB and ELISA - O96011

Core Product Specifications and Parameters

Biological Background: PEX11B Function and Localization

• PEX11B (Peroxisomal membrane protein 11B), also known as Peroxin-11B or PEX11-beta, is a peroxin that plays a central role in peroxisomal proliferation.
  Related references: PMID:9792670
• It may regulate peroxisomal division by recruiting the dynamin-related GTPase DNM1L to the peroxisomal membrane.
  Related references: PMID:12618434
• PEX11B promotes membrane protrusion and elongation on the peroxisomal surface, a prerequisite for fission.
  Related references: PMID:20826455
• The protein localizes to the peroxisome membrane and contains transmembrane domains.
• Post-translational modifications include acetylation, and alternative splicing generates multiple isoforms.
• Mutations in PEX11B are linked to peroxisome biogenesis disorders, underscoring its essential role in peroxisomal homeostasis.

Experimental Guidance and Technical Tips

• This polyclonal antibody is raised against amino acids 100-180 of human PEX11B; it may detect different isoforms and is expected to recognize the target in denaturing and native conditions.
• For Western blot (WB), consider using peroxisome-enriched fractions and validate the band size around 28 kDa; titrate the antibody for optimal signal.
• The antibody cross-reacts with mouse and rat PEX11B, making it suitable for comparative studies in mammalian models.
• In ELISA, optimize the coating antigen and antibody dilution to ensure specificity; the recombinant immunogen can serve as a positive control.
• Due to the polyclonal nature, batch-to-batch variability may occur; include appropriate controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, from antibody pairs to bulk reagents. For peroxisome biology and related disorders, we offer validated polyclonal antibodies like this anti-PEX11B, as well as monoclonal options and ancillary proteins. Our platform streamlines sourcing, technical consultation, and supply chain management to accelerate your concept-to-clinic journey.