Anti-PEX10 Rabbit Polyclonal Antibody for WB and ELISA - O60683

Core Product Specifications and Parameters

Biological Background: PEX10 Function and Localization

• PEX10, also known as peroxisome biogenesis factor 10, peroxin-10, peroxisomal biogenesis factor 10, peroxisome assembly protein 10, and RING finger protein 69, is a key component of the peroxisomal protein import machinery.
• It functions as an E3 ubiquitin-protein ligase within a retrotranslocation channel that exports the PEX5 receptor from peroxisomes to the cytosol, facilitating PEX5 recycling. Related references: PMID:24662292
• The retrotranslocation channel is assembled from PEX2, PEX10, and PEX12, with each subunit contributing transmembrane segments that form an open channel specific for PEX5 passage.
• When PEX5 recycling is impaired, PEX10 catalyzes the polyubiquitination of PEX5 during its transit through the channel, targeting it for degradation.
• PEX10 is localized to the peroxisome membrane and possesses a zinc-finger domain typical of RING-type E3 ligases, essential for its ubiquitination activity.
• Mutations in PEX10 are associated with peroxisome biogenesis disorders such as Zellweger syndrome, underscoring its critical role in peroxisomal function and human health.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 10-160 of human PEX10, which may confer reactivity to epitopes within this region; consider this when designing blocking or competition experiments.
• As a polyclonal antibody, it inherently recognizes multiple epitopes, potentially offering enhanced signal in WB and ELISA assays compared to monoclonal antibodies.
• For WB, validate the antibody in relevant peroxisome-containing cell lines (e.g., HepG2) under reducing conditions, given the predicted molecular weight of approximately 37 kDa.
• In ELISA, consider using the recombinant immunogen as a positive control to assess antibody binding specificity and titer.
• Cross-reactivity with mouse has been reported, enabling translational studies in mouse models of peroxisomal disorders, but always confirm with appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic and research communities with comprehensive IVD raw-material solutions, including validated polyclonal antibodies like this anti-PEX10 antibody for peroxisome biology studies. With our extensive portfolio of antibody pairs, monoclonal/polyclonal options, and bulk ancillary reagents, we streamline procurement for assays targeting rare or specialized targets. Count on CamelBio to bridge your project from discovery to diagnostic development with reliable, research-use materials.