Anti-PERP Rabbit Monoclonal Antibody for WB, IHC, IF - Q96FX8

Core Product Specifications and Parameters

Biological Background: PERP Function and Localization

• PERP (p53 apoptosis effector related to PMP-22), also known as keratinocyte-associated protein 1 (KCP-1), P53-induced protein PIGPC1, and transmembrane protein THW, is a component of intercellular desmosome junctions.
• Plays a critical role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly, thereby contributing to the barrier function of the skin and mammary tissue homeostasis.
• Required for tooth enamel development via facilitating desmosome-mediated ameloblast adhesion and may regulate downstream genes such as AMBN, ENAM, MMP20, and KLK4.
• Serves as an effector in the TP53-dependent apoptotic pathway and positively regulates apoptosis in T-helper 17 (Th17) cell populations through caspase-dependent signaling.
• Promotes neutrophil transepithelial migration in response to chemoattractants like HXA3, fMLP, and CXCL8, and is required for neutrophil migration during S. typhimurium infection. Related references: PMID:25486861
• Localizes to the cell membrane, desmosome cell junctions, and cytoplasm.
• Expressed in skin, heart, placental, liver, pancreas, keratinocytes, and dermal fibroblasts; may translocate to intestinal epithelial cell surface during Salmonella infection. Related references: PMID:25486861 PMID:27078059
• Associated with disease variants linked to palmoplantar keratoderma; annotated with keywords including apoptosis, cell adhesion, and cell junction.

Experimental Guidance and Technical Tips

• For Western blotting, consider using the predicted molecular weight of ~21 kDa as a reference; optimize loading and detection conditions for your sample.
• In immunohistochemistry (IHC) and immunofluorescence (IF), validate permeabilization and antigen retrieval methods suitable for desmosome-associated membrane proteins.
• This antibody has been tested in human, mouse, and rat samples; cross-reactivity in other species should be verified individually.
• As a monoclonal antibody produced in rabbit, it can be used with standard anti-rabbit secondary detection reagents; ensure proper isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials like the PERP antibody, covering apoptosis and cell adhesion targets. We offer validated antibody pairs, bulk monoclonal antibodies, and ancillary reagents to accelerate your assay development from concept to clinic. Contact us for custom sourcing of rare targets and technical support.