Anti-Peroxiredoxin 6 Rabbit Monoclonal Antibody (KD Validated) for WB, IHC-P, ELISA - P30041

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin-6 Function and Localization

• Peroxiredoxin-6 (PRDX6) is a bifunctional enzyme that possesses both thiol-specific peroxidase activity, reducing hydrogen peroxide and organic hydroperoxides, and calcium-independent phospholipase A2 activity, hydrolyzing the sn-2 ester bond of phospholipids. Related references: PMID:10893423 PMID:9497358 PMID:26830860
• It protects cells against oxidative stress by detoxifying a wide range of peroxides, including short chain organic, fatty acid, and phospholipid hydroperoxides. Related references: PMID:10893423
• PRDX6 also exhibits glutathione-dependent peroxidase activity and is alternatively known as non-selenium glutathione peroxidase (NSGPx).
• The enzyme functions as a lysophosphatidylcholine acyltransferase (LPCAT-5), preferentially converting lysophosphatidylcholine to phosphatidylcholine using palmitoyl CoA, playing a key role in phospholipid homeostasis. Related references: PMID:26830860
• Subcellularly, PRDX6 localizes to both the cytoplasm and lysosomes, linking its antioxidant and lipid metabolic functions.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomic studies.
• It is a member of the peroxiredoxin family, specifically classified as a 1-Cys peroxiredoxin, with a conserved cysteine residue (Cys47) forming the redox-active center.
• PRDX6 integrates antioxidant defense with lipid degradation and metabolism, contributing to membrane lipid homeostasis and cellular protection.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human PRDX6 protein, suggesting the antibody may recognize both native and denatured forms; consider optimizing dilutions for each specific application.
• For Western blotting, a band at approximately 25 kDa is expected; validate using appropriate positive control lysates (e.g., human or rat tissues known to express PRDX6).
• For immunohistochemistry-paraffin (IHC-P), include antigen retrieval steps and confirm staining specificity with known PRDX6-expressing tissues.
• ELISA performance should be optimized by checkerboard titration to establish the best coating and detection concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw material procurement with comprehensive one-stop access to validated antibodies, bulk ancillary reagents, and rare target sourcing—supporting every stage from concept to clinic. This anti-PRDX6 monoclonal antibody exemplifies our commitment to delivering high-quality tools for oxidative stress and lipid metabolism research. Whether you need optimized antibody pairs or tailored sourcing for niche targets, CamelBio is your reliable partner for diagnostic development.