Anti-Peroxiredoxin 6 Polyclonal Antibody for WB - P30041

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin 6 Function and Localization

• Peroxiredoxin-6 (PRDX6) is a 25 kDa multifunctional enzyme belonging to the peroxiredoxin family, also known as 1-Cys peroxiredoxin, antioxidant protein 2 (AOP2), and non-selenium glutathione peroxidase (NSGPx). Its gene symbol is PRDX6.
• Catalyzes the reduction of hydrogen peroxide and organic hydroperoxides (including short chain, fatty acid, and phospholipid hydroperoxides) to water and alcohols, playing a crucial role in cellular defense against oxidative stress. Related references: PMID:10893423, PMID:9497358
• Exhibits calcium-independent phospholipase A2 (aiPLA2) activity, hydrolyzing the sn-2 ester bond of phospholipids; this activity is pH-dependent and contributes to phospholipid homeostasis. Related references: PMID:10893423, PMID:26830860
• Functions as lysophosphatidylcholine acyltransferase 5 (LPCAT-5), preferentially converting lysophosphatidylcholine into phosphatidylcholine using palmitoyl CoA as an acyl donor. Related references: PMID:26830860
• Predominantly localized in the cytoplasm and lysosomes.
• Contains a redox-active cysteine residue (Cys47) essential for peroxidase activity; subject to acetylation and phosphorylation, as identified by proteomics. The 3D structure has been determined.
• Classified as an antioxidant, hydrolase, oxidoreductase, peroxidase, and transferase, emphasizing its multifunctional enzymatic nature.

Experimental Guidance and Technical Tips

• For western blotting, run samples under reducing conditions and expect a band at approximately 25 kDa; validate detection in lysates from human, mouse, or rat tissues/cell lines based on reported cross-reactivity.
• In immunocytochemistry/immunofluorescence (IF/ICC), anticipate cytoplasmic and punctate lysosomal staining; include appropriate fixation and permeabilization steps, and use isotype controls.
• For immunohistochemistry (IHC-P), optimize antigen retrieval (e.g., heat-induced epitope retrieval) and blocking steps; confirm specificity with tissue-specific controls and pre-immune serum if available.
• Since the antibody is generated against full-length recombinant protein, it may recognize both reduced and oxidized forms; confirm the redox state relevance in your experimental system.
• The antibody is suitable for ELISA; pair with a matched detection antibody or standard protein for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop supplier of IVD raw materials, serving diagnostic manufacturers and research institutions worldwide. For oxidative stress research and related assay development, we offer a comprehensive portfolio including validated polyclonal antibodies against Peroxiredoxin 6, matched antibody pairs, and complementary reagents. Our flexible sourcing solutions support projects from early-stage research to clinical validation, with bulk supply and customized services available.