Anti-Peroxiredoxin 6 Monoclonal Antibody for WB, ELISA - P30041

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin-6 Function and Localization

• Peroxiredoxin-6 (PRDX6), also known as 1-Cys peroxiredoxin, AOP2, NSGPx, or aiPLA2, is a member of the peroxiredoxin family of antioxidant enzymes.
• It functions as a thiol-specific peroxidase, reducing hydrogen peroxide and organic hydroperoxides to water and alcohols, thereby protecting cells from oxidative damage. Related references: PMID:10893423, PMID:9497358
• PRDX6 can also reduce phospholipid hydroperoxides, playing a direct role in repairing oxidatively damaged cell membranes. Related references: PMID:10893423
• In addition to peroxidase activity, it possesses phospholipase A2 (PLA2) activity, hydrolyzing the sn-2 ester bond of phospholipids, which is regulated by pH and lipid binding. Related references: PMID:10893423, PMID:26830860
• PRDX6 also exhibits lysophospholipid acyltransferase activity, catalyzing the conversion of lysophosphatidylcholine to phosphatidylcholine using palmitoyl-CoA as a preferred acyl donor. Related references: PMID:26830860
• Localized predominantly in the cytoplasm and lysosomes, PRDX6 is a multifunctional enzyme involved in antioxidant defense and phospholipid metabolism.
• Post-translational modifications such as acetylation and phosphorylation have been identified, and the protein contains a redox-active cysteine residue critical for its peroxidase function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 125-224 of human PRDX6, targeting the C-terminal region. This design is expected to recognize full-length PRDX6 in denaturing conditions suitable for Western blot.
• For Western blot, validate detection of a ~25 kDa band in whole cell lysates from human, mouse, or rat tissues, taking advantage of the reported cross-reactivity.
• For ELISA, consider using this monoclonal antibody as a capture or detection reagent after optimizing with recombinant PRDX6 protein or native samples.
• Include appropriate controls, such as PRDX6 knockdown or knockout cell lines, to confirm band specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and labs with streamlined access to high-quality IVD raw materials tailored to Peroxiredoxin-6 research, including validated monoclonal antibodies, antigen-grade proteins, and ancillary reagents. Our sourcing network delivers optimized antibody pairs and bulk supplies to support assay development for oxidative stress biomarkers. From concept to clinic, CamelBio ensures reliable supply chain solutions for your Peroxiredoxin-6-related immunoassay needs.