Anti-Peroxiredoxin 5 (PRDX5) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P30044

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin 5 Function and Localization

• Peroxiredoxin-5 (PRDX5, also known as PLP, ACR1, AOEB166, PMP20, Prx-V) is a member of the peroxiredoxin family of thiol-specific antioxidant enzymes.
• It catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, thereby protecting cells from oxidative stress and participating in redox signaling.
• The PRDX5 gene undergoes alternative splicing and alternative initiation, producing multiple isoforms with distinct subcellular localizations.
• The protein is widely expressed across tissues and localizes to mitochondria, cytoplasm, and peroxisomes, reflecting its role in scavenging peroxides from various cellular compartments.
• Post-translational modifications include acetylation, palmitoylation, and phosphorylation, which may regulate its activity, stability, or subcellular targeting.
• PRDX5 is classified as an antioxidant enzyme with a redox-active cysteine residue that forms a disulfide bond during its catalytic cycle.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein spanning amino acids 53-214, a region conserved across human, mouse, and rat PRDX5; it is therefore expected to detect the target in these species.
• For Western blot, a band at ~22 kDa is anticipated; consider using whole-cell lysates or mitochondrial-enriched fractions to assess expression.
• In immunofluorescence/ICC, localization to mitochondria, peroxisomes, or cytoplasm may be observed; co-staining with organelle markers is recommended.
• ELISA can be performed using the immunogen or native protein; titration experiments are advised to optimize antibody dilution.
• As a polyclonal antibody, lot-to-lot consistency should be verified; always validate in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive raw-material sourcing for IVD manufacturers and research laboratories, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. With this anti-PRDX5 antibody, we support studies of oxidative stress and antioxidant defense mechanisms, from concept to clinical application. Our team assists in rare-target reagent procurement, ensuring reliable supply chains for your diagnostic development needs.