Anti-Peroxiredoxin 5 (PRDX5) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P30044

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin-5 Function and Localization

• Encoded by the PRDX5 gene, Peroxiredoxin-5 (PRDX5) is a thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.
• The protein plays a critical role in cellular defense against oxidative stress by detoxifying peroxides and also functions as a sensor of hydrogen peroxide-mediated signaling events.
• Also known as ACR1, B166, PRXV, PMP20, PRDX6, prx-V, SBBI10, AOEB166, and HEL-S-55, it belongs to the peroxiredoxin family of antioxidant enzymes.
• PRDX5 localizes to multiple subcellular compartments including the mitochondrion, cytoplasm, and peroxisome matrix.
• Widely expressed across tissues, PRDX5 is a ubiquitous antioxidant enzyme.
• Post-translational modifications include acetylation, phosphorylation, and palmitoylation, and it exists as a lipoprotein with a redox-active catalytic cysteine.
• Alternative initiation and alternative splicing generate multiple isoforms, as supported by proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region encompassing the core catalytic domain, which is conserved across human, mouse, and rat (consistent with cross-reactivity data).
• For Western blot, a band at ~22 kDa is expected. Validate the antibody in your sample system using appropriate positive and negative controls.
• For immunofluorescence/immunocytochemistry, consider optimal fixation and permeabilization conditions to detect mitochondrial, cytoplasmic, or peroxisomal localization.
• For ELISA, the antibody may be used for antigen detection or capture, but titers and conditions should be optimized experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For PRDX5 and oxidative stress research, we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your development workflow. Let CamelBio be your trusted partner in accelerating diagnostics from bench to market.