Anti-Peroxiredoxin 5 (PRDX5) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P30044

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin 5 (PRDX5) Function and Localization

• Encodes Peroxiredoxin-5, a mitochondrial, cytosolic, and peroxisomal antioxidant enzyme also known as PLP, ACR1, PMP20, and AOEB166.
• Thiol-specific peroxidase that reduces hydrogen peroxide and organic hydroperoxides to water and alcohols, protecting cells against oxidative stress and serving as a peroxide sensor.
• Belongs to the peroxiredoxin family and contains a redox-active cysteine residue critical for enzymatic activity.
• Localizes to the mitochondrial matrix, cytoplasm, and peroxisome matrix, reflecting its role in multiple subcellular compartments.
• Subject to post-translational modifications including acetylation, phosphorylation, and palmitoylation, which may regulate its function.
• Widely expressed across human tissues, consistent with its fundamental role in antioxidant defense.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 53-214, covering a substantial portion of the mature protein; this region is expected to detect all known isoforms of PRDX5.
• For Western blotting, the predicted molecular weight is 22 kDa; validate band specificity using knockout controls where possible.
• For immunohistochemistry, consider antigen retrieval methods and verify signal localization in mitochondria, cytoplasm, and peroxisomes.
• ELISA applications may require optimization of coating conditions and antibody pairings for best sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a seamless sourcing experience for IVD raw materials. Our portfolio includes validated antibodies like this anti-PRDX5 monoclonal antibody, ensuring performance for oxidative stress and antioxidant research. From concept to clinic, we support your projects with bulk reagents, technical consulting, and tailored sourcing solutions.