Anti-Peroxiredoxin 5 (PRDX5) Rabbit Monoclonal Antibody for WB, ELISA - P30044

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin-5 Function and Localization

• Peroxiredoxin-5, mitochondrial (PRDX5) is a thiol-specific peroxidase belonging to the peroxiredoxin family. It is also known as Alu corepressor 1, antioxidant enzyme B166, peroxiredoxin V, and thioredoxin peroxidase PMP20.
• Catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively, thereby protecting cells against oxidative stress and participating in peroxide-mediated signaling.
• PRDX5 functions as both a detoxifier of peroxides and a sensor of hydrogen peroxide-mediated signaling events, contributing to redox homeostasis.
• Localizes to multiple subcellular compartments: mitochondrion, cytoplasm, and peroxisome matrix, indicating versatile roles in organellar antioxidant defense.
• Widely expressed across tissues, underscoring its fundamental role in cellular protection.
• Post-translational modifications include acetylation, phosphorylation, and palmitoylation, which may regulate its activity, localization, or interactions. Alternative initiation and alternative splicing generate multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 100-200 of human PRDX5 (P30044), which is expected to recognize endogenous PRDX5 in relevant model systems.
• For Western blotting, consider a protein band of approximately 22kDa under reducing conditions; validate the observed molecular weight in your specific sample type.
• Given the reported cross-reactivity with human, mouse, and rat, this antibody can be applied to comparative studies across these species; preliminary verification in your laboratory is recommended.
• In ELISA applications, assess optimal antibody dilution and coating conditions to ensure specific detection of PRDX5 in serum, plasma, or cell lysate samples.
• PRDX5 expression may vary under oxidative stress conditions; include appropriate positive and negative controls to confirm detection specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, laboratories, and research institutions to deliver a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Targeting oxidative stress and antioxidant enzymes, this PRDX5 antibody exemplifies our capability to supply reagents for key research areas. Whether you need ready-to-use reagents or custom rare-target sourcing, CamelBio provides end-to-end support from concept to clinic.