Anti-Peroxiredoxin 4 (PRDX4) Rabbit Polyclonal Antibody for WB, ELISA - Q13162

Core Product Specifications and Parameters

Biological Background: PRDX4 Function and Localization

• Peroxiredoxin-4 (PRDX4) is a thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, protecting cells against oxidative stress.
• It functions as a sensor of hydrogen peroxide-mediated signaling events, contributing to cellular redox regulation.
• PRDX4 regulates the activation of NF-kappa-B in the cytosol by modulating I-kappa-B-alpha phosphorylation, linking oxidative stress to inflammatory signaling.
• The protein localizes to both the cytoplasm and the endoplasmic reticulum, indicating roles in multiple subcellular compartments.
• It belongs to the peroxiredoxin family and contains a redox-active cysteine residue that forms a disulfide bond during the catalytic cycle.
• Alternative names include antioxidant enzyme AOE372, thioredoxin peroxidase AO372, and peroxiredoxin IV, reflecting its antioxidant and peroxidase activities.
• The enzyme is involved in detoxifying peroxides, contributing to overall cellular homeostasis and defense mechanisms.

Experimental Guidance and Technical Tips

• For Western blot applications, the predicted molecular weight of PRDX4 is approximately 31 kDa; consider using appropriate molecular weight markers and loading controls.
• The polyclonal antibody was raised against a recombinant protein spanning amino acids 38–271 of human PRDX4, which covers the majority of the mature protein; it may detect both reduced and oxidized forms.
• Given the cross-reactivity with mouse and rat, validate the antibody in the relevant species sample system if using these models.
• For ELISA, consider titrating the antibody to determine optimal working concentrations for your specific assay conditions.
• The antibody is validated for WB and ELISA; additional applications should be tested empirically for suitability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, streamlining your procurement from discovery to diagnostic development. With expertise in oxidative stress and antioxidant enzyme targets, we support your research and assay needs with high-quality, ready-to-use reagents. As your sourcing partner, we help accelerate your project from concept to clinic with a one-stop solution for all raw-material requirements.