Anti-Peroxiredoxin 4 (PRDX4) Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13162

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin 4 Function and Localization

• Peroxiredoxin-4 (PRDX4), also known as PRX-4, AOE372, or AOE37-2, is a thiol-specific peroxidase belonging to the peroxiredoxin family.
• It catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, playing a critical role in cellular protection against oxidative stress.
• PRDX4 functions as a sensor of hydrogen peroxide-mediated signaling events and regulates the activation of NF-kappa-B by modulating I-kappa-B-alpha phosphorylation.
• The protein localizes to both the cytoplasm and the endoplasmic reticulum, enabling peroxide detoxification in multiple subcellular compartments.
• PRDX4 contains a redox-active cysteine residue that forms a disulfide bond during its catalytic cycle, characteristic of typical 2-Cys peroxiredoxins.
• Key post-translational modifications and structural features include the active-site cysteine and disulfide bond formation, essential for its peroxidase activity.
• It is also annotated as an antioxidant enzyme, with proteomics and direct protein sequencing data confirming its expression (UniProt keywords: Antioxidant, Oxidoreductase, Peroxidase, Redox-active center).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 10-110 of human PRDX4, which may yield detection of both endogenous and recombinant protein.
• For Western blot, a band around 31 kDa is expected; consider using appropriate positive controls such as HeLa or HepG2 cell lysates.
• For IHC-P, antigen retrieval and antibody titration are recommended; validate in tissues known to express PRDX4.
• Species cross-reactivity with mouse and rat allows application in common animal models; verify reactivity in your specific sample type.
• For IF/ICC, confirm localization to cytoplasm and endoplasmic reticulum using appropriate markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to high-quality IVD raw materials. For studies on oxidative stress and redox signaling, we offer validated antibodies like this anti-PRDX4 polyclonal antibody, along with optimized antibody pairs and ancillary reagents. Our sourcing services cover rare and specialized targets, ensuring your projects move from concept to clinic efficiently.