Anti-Peroxiredoxin 4 (PRDX4) Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13162

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin 4 Function and Localization

• Peroxiredoxin-4 (PRDX4), also known as antioxidant enzyme AOE372, thioredoxin peroxidase AO372, or Prx-IV, belongs to the peroxiredoxin family of thiol-specific peroxidases.
• It catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively, playing a key role in cellular defense against oxidative stress.
• PRDX4 also functions as a sensor of hydrogen peroxide-mediated signaling, regulating the activation of NF-kappa-B by modulating I-kappa-B-alpha phosphorylation in the cytosol.
• Encoded by the PRDX4 gene (GeneID: 10549), the protein is localized primarily in the cytoplasm and the endoplasmic reticulum.
• PRDX4 contains a redox-active cysteine residue that undergoes cycles of oxidation and reduction, forming a disulfide bond during its catalytic cycle.
• Keywords associated with PRDX4 include antioxidant, oxidoreductase, peroxidase, redox-active center, and reference proteome, highlighting its central role in maintaining cellular redox homeostasis.
• The mature protein has a calculated molecular weight of ~30.5 kDa, consistent with the observed ~31 kDa band.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 1–100 of human Peroxiredoxin 4. This N-terminal region is conserved; cross-reactivity with mouse and rat is reported, but validation in the specific experimental system is recommended.
• For Western blotting, the predicted molecular weight is ~31 kDa; consider using a reducing gel and standard loading controls to confirm specificity.
• In immunohistochemistry (IHC-P), antigen retrieval conditions may need optimization for different tissue types; positive and negative controls are advised.
• For ELISA, pairing this monoclonal antibody with a detection antibody could be explored for sandwich ELISA development; however, epitope compatibility must be verified.
• For immunofluorescence (IF/ICC), fixation and permeabilization protocols should be tested to ensure proper antigen accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, providing diagnostic manufacturers, labs, and research institutes with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing from concept to clinic. This anti-PRDX4 monoclonal antibody supports oxidative stress and cell signaling research. Contact us to explore tailored supply solutions for your IVD development needs.