Anti-Peroxiredoxin 3 (PRDX3) Rabbit Polyclonal Antibody for WB, ELISA - P30048

Core Product Specifications and Parameters

Biological Background: PRDX3 Function and Localization

• Peroxiredoxin 3 (PRDX3), also known as AOP‑1, belongs to the peroxiredoxin family of antioxidant enzymes and is the mitochondrial member of this family. It functions as a thioredoxin‑dependent peroxidase that reduces hydrogen peroxide and organic hydroperoxides to water and alcohols, thereby protecting cells from oxidative stress. Related references: PMID:17707404 PMID:29438714 PMID:33889951
• PRDX3 interacts with MAP3K13 to synergistically regulate NF‑kappa‑B activation in the cytosol, linking redox signaling to inflammatory pathways. Related references: PMID:12492477
• Required for the maintenance of physical strength, as demonstrated by similarity to other species.
• Primarily localizes to the mitochondrial matrix, with additional presence detected in the cytoplasm and early endosomes.
• Subject to multiple post‑translational modifications, including acetylation, palmitoylation, phosphorylation, and disulfide bond formation; contains a conserved redox‑active cysteine center critical for its peroxidase activity.
• Mutations in the PRDX3 gene are associated with spinocerebellar ataxia type 32 (SCAR32) and corneal dystrophy, highlighting its role in neurodegeneration and oxidative stress‑related pathologies.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody corresponds to amino acids 63–256 of human PRDX3 (NP_006784.1), spanning the mature protein after mitochondrial import and processing. For Western blot (WB) analysis, consider using whole‑cell or mitochondrial‑enriched lysates from human or rat cells expressing endogenous PRDX3. Under reducing conditions, the expected band is approximately 28 kDa.
• For ELISA applications, the antibody can be employed as a capture or detection reagent; optimization of coating concentration and detection antibody pairings is recommended for best sensitivity.
• When working with mitochondrial proteins, ensure proper sample preparation (e.g., inclusion of protease inhibitors and avoidance of excessive heat denaturation) to maintain epitope integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material sourcing, offering this rabbit anti‑PRDX3 polyclonal antibody alongside a comprehensive range of validated antibody pairs, recombinant proteins, and ancillary reagents. Whether you are developing assays for oxidative stress, mitochondrial dysfunction, or neurodegenerative biomarkers, our one‑stop supply chain streamlines your procurement from discovery to clinical manufacturing. Our dedicated technical support team is available to assist with custom requirements and bulk orders.