Anti-Peroxiredoxin 3 (PRDX3) Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P30048

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin 3 (PRDX3) Function and Localization

• Thioredoxin-dependent peroxidase that reduces hydrogen peroxide and organic hydroperoxides, protecting cells from oxidative stress. Related references: PMID:17707404 PMID:29438714 PMID:33889951
• Acts synergistically with MAP3K13 to regulate NF-kappa-B signaling in the cytosol. Related references: PMID:12492477
• Localizes primarily to mitochondria, but also found in cytoplasm and early endosomes.
• Member of the peroxiredoxin family, containing a redox-active cysteine center.
• Subject to post-translational modifications including acetylation, phosphorylation, and palmitoylation.
• Genetic variants are associated with corneal dystrophy and neurodegenerative disorders.
• Required for the maintenance of physical strength (by similarity).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the C-terminal part of PRDX3; consider this when designing detection and blocking strategies.
• For Western blotting, PRDX3 migrates at an apparent molecular weight of ~28 kDa under reducing conditions; ensure proper gel percentage for resolution.
• Validate specificity in relevant species (human, mouse) and consider potential cross-reactivity with other peroxiredoxin family members due to sequence homology.
• For immunohistochemistry, mitochondrial localization may require antigen retrieval optimization.
• In IF/ICC, co-localization studies with mitochondrial markers can confirm subcellular distribution.
• For ELISA, consider using recombinant PRDX3 protein as a standard to assess antibody binding affinity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering seamless access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. Our PRDX3 antibody supports research into oxidative stress and mitochondrial disorders, backed by a supply chain that ensures consistency from concept to clinic.