Anti-Peroxiredoxin 2 (PRDX2) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P32119

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin-2 Function and Localization

• Peroxiredoxin-2 (PRDX2), also known as natural killer cell-enhancing factor B (NKEF-B) or thioredoxin peroxidase 1, is a ~22 kDa member of the peroxiredoxin family of antioxidant enzymes.
• It functions as a thiol-specific peroxidase, catalyzing the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.
• PRDX2 protects cells against oxidative stress by detoxifying peroxides and acts as a sensor in hydrogen peroxide-mediated signaling events.
• The protein has been implicated in the signaling cascades of growth factors and tumor necrosis factor-alpha by modulating intracellular hydrogen peroxide concentrations.
• PRDX2 is primarily localized in the cytoplasm, where it exerts its antioxidant functions.
• It undergoes post-translational modifications including acetylation and phosphorylation, and contains a redox-active disulfide bond essential for its catalytic activity.
• Alternative splicing of the PRDX2 gene results in multiple transcript variants, contributing to functional diversity.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band near 22 kDa; reducing conditions are recommended to break any disulfide-linked dimers or oligomers.
• In immunofluorescence/ICC, cytoplasmic staining is anticipated; fixation and permeabilization protocols should be optimized for the target cell type.
• Validate antibody specificity using a positive control lysate from cells with known PRDX2 expression, such as HeLa or Jurkat.
• Cross-reactivity with mouse and rat has been reported, but sequence alignment of the immunogen region is advised to confirm reactivity in these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-PRDX2 antibody supports research into oxidative stress and redox signaling, and CamelBio can assist with sourcing rare-target raw materials and providing tailored technical support to accelerate your assay development from concept to clinic.