Anti-Peroxiredoxin 2 (PRDX2) Polyclonal Antibody for WB, IF/ICC, ELISA - P32119

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin-2 Function and Localization

• Peroxiredoxin-2 (PRDX2), encoded by the PRDX2 gene, is also known as natural killer cell-enhancing factor B (NKEF-B), thiol-specific antioxidant protein (TSA), and thioredoxin peroxidase 1.
• Functions as a thiol-specific peroxidase that reduces hydrogen peroxide and organic hydroperoxides to water and alcohols, thereby protecting cells from oxidative stress.
• Acts as a sensor and regulator of hydrogen peroxide-mediated intracellular signaling, potentially participating in growth factor and tumor necrosis factor-alpha cascades.
• Belongs to the peroxiredoxin family of antioxidant enzymes, characterized by a conserved redox-active cysteine residue that forms a disulfide bond during the catalytic cycle.
• Localizes predominantly to the cytoplasm, consistent with its role in detoxifying reactive oxygen species generated in various cellular compartments.
• Subject to post-translational modifications including acetylation and phosphorylation, which may regulate its activity or protein interactions.
• Keywords from UniProt: antioxidant, oxidoreductase, peroxidase, cytoplasm, disulfide bond, phosphoprotein, acetylation, and alternative splicing.

Experimental Guidance and Technical Tips

• For Western blot, use reducing conditions to resolve the monomeric ~22 kDa band; note that PRDX2 can form disulfide-linked dimers under non-reducing conditions.
• In immunofluorescence/immunocytochemistry, fix and permeabilize cells appropriately to detect the cytoplasmic localization of PRDX2; consider using specific organelle markers to confirm subcellular distribution.
• For ELISA, pairing with a suitable capture antibody and using the recombinant immunogen as a standard can help establish a sensitive and specific detection system.
• Validate antibody reactivity in the relevant sample system (e.g., cell lines or tissues from human, mouse, or rat) to ensure consistent performance across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. Through our extensive supply network, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-PRDX2 antibody, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your project in oxidative stress and antioxidant defense research. Contact us to discuss your specific requirements and streamline your procurement process.