Anti-Peroxiredoxin 2 (PRDX2) Monoclonal Antibody for WB and ELISA - P32119

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin 2 (PRDX2) Function and Localization

• Peroxiredoxin-2 (PRDX2) is a member of the peroxiredoxin family of antioxidant enzymes, encoded by the PRDX2 gene (also known as NKEFB and TDPX1). It is also referred to as natural killer cell-enhancing factor B (NKEF-B), thiol-specific antioxidant protein (TSA), and thioredoxin peroxidase 1.
• PRDX2 functions as a thiol-specific peroxidase, catalyzing the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively, thereby protecting cells against oxidative stress.
• By detoxifying peroxides, PRDX2 also acts as a sensor of hydrogen peroxide-mediated signaling events and may participate in signaling cascades involving growth factors and tumor necrosis factor-alpha by regulating intracellular H2O2 concentrations.
• The protein primarily localizes to the cytoplasm, where it carries out its redox functions.
• PRDX2 undergoes various post-translational modifications, including acetylation, phosphorylation, and disulfide bond formation at its redox-active center, which may modulate its activity and interactions.
• The PRDX2 gene is subject to alternative splicing, potentially generating multiple isoforms with distinct functions or regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human PRDX2, targeting the N-terminal region. Ensure the antigen sequence is conserved in your species of interest; this antibody cross-reacts with mouse and rat PRDX2.
• For Western blot, a band at approximately 22 kDa is expected. Optimize antibody dilution using a known positive control sample and confirm band identity with a peptide competition assay if needed.
• For ELISA applications, consider using the recombinant protein or the immunizing peptide as a coating antigen to establish assay conditions and determine appropriate antibody concentrations.
• Because PRDX2 is an abundant cytoplasmic protein involved in redox regulation, sample preparation should minimize oxidation artifacts; include reducing agents in lysis buffer and consider running non-reducing vs reducing gels to detect redox-dependent modifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents. Our Peroxiredoxin 2 (PRDX2) monoclonal antibody supports oxidative stress and redox biology research, and we offer one-stop sourcing for all related raw materials—from bulk antibodies to rare antigen preparations. With a focus on bridging concept to clinic, CamelBio streamlines your supply chain for immunodiagnostic development.