Anti-Peroxiredoxin 1/PAG Rabbit mAb for WB, ELISA - Q06830

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin 1/PAG Function and Localization

• Also known as Natural killer cell-enhancing factor A (NKEF-A), Proliferation-associated gene protein (PAG), Thioredoxin peroxidase 2, and Thioredoxin-dependent peroxide reductase 2. It belongs to the peroxiredoxin family and is encoded by the PRDX1 gene in humans.
• Functions as a thiol-specific peroxidase that reduces hydrogen peroxide and organic hydroperoxides to water and alcohols, playing a critical role in cellular antioxidant defense.
• Acts as a sensor of hydrogen peroxide-mediated signaling events and may participate in growth factor and tumor necrosis factor-alpha signaling cascades by regulating intracellular H2O2 concentrations.
  Related references:
  PMID:9497357
• Localizes primarily to the cytoplasm and also to melanosomes, as indicated by subcellular localization data.
• Post-translationally modified by acetylation, phosphorylation, and ubiquitin-like conjugation, and features a redox-active center with disulfide bond formation, essential for its catalytic cycle.
• Reported to reduce an intramolecular disulfide bond in GDPD5, which gates the ability of GDPD5 to drive postmitotic motor neuron differentiation (by similarity).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human Peroxiredoxin 1 (Q06830). This antibody is expected to recognize the full-length native protein.
• For Western blotting, a band of approximately 22 kDa is anticipated. Consider using appropriate positive controls (e.g., human or mouse cell lysates known to express PRDX1) and optimize antibody dilutions experimentally.
• In ELISA, this antibody can be used as a capture or detection reagent when paired with a compatible secondary antibody or HRP conjugate. Validate in your specific sample matrix and include appropriate standards.
• Cross-reactivity is reported for human and mouse, but reactivity with other species should be verified if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, providing comprehensive access to validated antibody pairs, bulk recombinant proteins, and ancillary reagents to accelerate diagnostic development. This anti-Peroxiredoxin 1 rabbit monoclonal antibody exemplifies our commitment to supply high-quality, application-tested monoclonal antibodies for oxidative stress and redox biology research. For customized sourcing, technical consultation, or large-volume requirements, our team is ready to support your project from concept to clinic.