Anti-Peroxiredoxin 1/PAG Polyclonal Antibody for WB and ELISA - Q06830

Core Product Specifications and Parameters

Biological Background: Peroxiredoxin 1 (PRDX1) Function and Localization

• Peroxiredoxin-1 (PRDX1) is a thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, serving as a key antioxidant enzyme. Related references: PMID:9497357
• It functions as a sensor of hydrogen peroxide-mediated signaling events and may modulate intracellular H2O2 concentrations to regulate growth factor and tumor necrosis factor-alpha signaling cascades. Related references: PMID:9497357
• Also known as Natural killer cell-enhancing factor A (NKEF-A), Proliferation-associated gene protein (PAG), Thioredoxin peroxidase 2, and Thioredoxin-dependent peroxiredoxin 1.
• The PRDX1 gene (GeneID: 5052) encodes a protein of 199 amino acids with a molecular weight of approximately 22 kDa.
• It is localized primarily in the cytoplasm and has been identified in melanosomes.
• Post-translational modifications include acetylation, phosphorylation, disulfide bond formation, and isopeptide bonds (ubiquitin-like conjugation), which are critical for its regulation.
• Reduces an intramolecular disulfide bond in GDPD5, gating its ability to drive postmitotic motor neuron differentiation (by similarity).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant full-length Peroxiredoxin-1 protein; therefore, the antibody may detect both reduced and oxidized forms. Consider using reducing and non-reducing SDS-PAGE conditions for Western blot to distinguish redox states.
• For ELISA, validate using recombinant Peroxiredoxin-1 protein as a positive control and optimize coating and detection conditions.
• Confirm antibody specificity in relevant human and mouse cell lines or tissue lysates, as cross-reactivity with these species is indicated.
• Given its role in oxidative stress, include appropriate antioxidant controls (e.g., NAC) when studying stimulus-induced peroxiredoxin modifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, technical services, and consulting. This Peroxiredoxin 1 antibody supports oxidative stress and cancer research, and we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare or custom targets, our team streamlines sourcing from discovery to clinical application.