Anti-PERK Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q9NZJ5

Core Product Specifications and Parameters

Biological Background: PERK (EIF2AK3) Function and Localization

• EIF2AK3/PERK is a metabolic-stress sensor kinase that, upon ER stress, autophosphorylates and phosphorylates eIF2-alpha (EIF2S1) at Ser51, attenuating global protein synthesis while inducing ATF4-mediated integrated stress response (ISR) gene expression. Related references:
  PMID:10026192
  PMID:10677345
  PMID:11907036
  PMID:27917829
• The PERK-ATF4 axis upregulates COX7A2L/SCAF1 to increase mitochondrial respiratory supercomplex formation and oxidative phosphorylation. Related references:
  PMID:31023583
• At mitochondria-ER contact sites, ATAD3A directly inhibits PERK, shielding mitochondria from UPR-mediated stress. Related references:
  PMID:39116259
• PERK also phosphorylates NRF2, triggering its dissociation from KEAP1, nuclear accumulation, and activation of antioxidant and cytoprotective genes.
• PERK localizes to the endoplasmic reticulum membrane, with a single-pass transmembrane topology and a luminal stress-sensing domain.
• Ubiquitously expressed, PERK is particularly abundant in secretory tissues such as pancreas, consistent with its role in ER proteostasis.
• Structurally, PERK is a glycoprotein, undergoes ADP-ribosylation, and contains a serine/threonine kinase domain with additional tyrosine kinase activity.
• Loss-of-function mutations in EIF2AK3 cause Wolcott-Rallison syndrome, characterized by neonatal diabetes and skeletal dysplasia.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal kinase domain of human PERK (residues 867–1116). This antibody is expected to detect total PERK levels regardless of phosphorylation state.
• For Western blot, protein lysates should be prepared under reducing conditions; a band near 125 kDa is expected. Low basal PERK expression may require ER stress induction with agents like tunicamycin or thapsigargin.
• In immunofluorescence and immunohistochemistry, consider co-staining with an ER marker (e.g., Calnexin) to confirm localization.
• For ELISA, consider using recombinant PERK protein or cell lysates from stressed cells as the capture/detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies and ancillary reagents. This PERK (EIF2AK3) monoclonal antibody, ideal for ER stress and UPR research, is part of our optimized collection for diagnostic and research applications. From bulk monoclonal antibody supply to rare-target sourcing, CamelBio supports the entire development pipeline.