Anti-Periostin Monoclonal Antibody for WB, ELISA - Q15063

Core Product Specifications and Parameters

Biological Background: Periostin Function and Localization

• Periostin is a secreted extracellular matrix protein involved in cell adhesion, inducing cell attachment and spreading. Related references: PMID:12235007
• Enhances incorporation of BMP1 into the fibronectin matrix, promoting proteolytic activation of lysyl oxidase LOX, contributing to connective tissue integrity.
• Localized to the Golgi apparatus and secreted into the extracellular space and matrix.
• Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid, and breast; also expressed in kidney and lung.
• Up-regulated in epithelial ovarian tumors, lung carcinoma periphery, and overexpressed in breast cancers; absent in normal ovaries.
• Alternative splicing produces multiple isoforms with molecular weights ranging from 80 to 93 kDa.
• Contains gamma-carboxyglutamic acid domains and heparin-binding sites, and is a glycoprotein with disulfide bonds.

Experimental Guidance and Technical Tips

• For Western blotting, consider using reducing conditions and a broad-range molecular weight marker to account for the multiple isoforms (80–93 kDa) and potential glycosylation.
• Validate the antibody in relevant human tissue or cell line lysates (e.g., aorta, breast cancer lines) to confirm specificity.
• For ELISA, consider coating with the immunogen peptide or recombinant Periostin, and optimize the antibody concentration for best signal-to-noise ratio.
• Due to the antibody's human specificity, it is not recommended for rodent samples unless cross-reactivity is independently confirmed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, labs, and research institutes with streamlined access to IVD raw materials, including this high-quality Periostin monoclonal antibody. As your one-stop partner, we also provide validated antibody pairs, optimized bulk monoclonals and polyclonals, ancillary reagents, and rare target sourcing—simplifying your journey from concept to clinic.