Anti-Perilipin A Monoclonal Antibody for WB, IHC-P, IF-P, ELISA - O60240

Core Product Specifications and Parameters

Biological Background: Perilipin-1 Function and Localization

• Perilipin-1, also known as PLIN1 or Lipid droplet-associated protein, is a key modulator of adipocyte lipid metabolism.
• It coats lipid storage droplets, protecting them from hormone-sensitive lipase (HSL)-mediated breakdown; its absence can lead to leanness.
• Perilipin-1 promotes unilocular lipid droplet formation by activating CIDEC, facilitating lipid droplet enlargement and directional neutral lipid transfer.
• The protein localizes primarily to lipid droplets and the endoplasmic reticulum.
• It is detected in adipocytes from white adipose tissue, visceral adipose tissue, and mammary gland. Related references: PMID:27832861 PMID:9521880
• As a phosphoprotein, Perilipin-1 is subject to post-translational modifications that may regulate its function.
• Additional keywords include lipid metabolism and proteomics identification.

Experimental Guidance and Technical Tips

• As the immunogen is a synthetic peptide from the central region (aa 400-500) of human Perilipin A, sequence alignment with mouse and rat orthologs is recommended to confirm expected cross-reactivity.
• In Western blotting, a band of approximately 56 kDa is expected; validate using appropriate positive controls such as adipocyte lysates.
• For IHC-P and IF-P, standard antigen retrieval and permeabilization methods may be used; optimization in the target tissue is advised.
• This rabbit monoclonal antibody offers high specificity and lot-to-lot consistency, suitable for quantitative ELISA applications.
• Always include proper negative controls to confirm specificity in the chosen application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For adipocyte biology and lipid metabolism research, this Perilipin A monoclonal antibody exemplifies the high-quality target-specific reagents available through CamelBio's supply network. From antibody procurement to technical support, CamelBio streamlines the path from concept to clinical application.