Anti-Perilipin-2 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P43883

Core Product Specifications and Parameters

Biological Background: Perilipin-2 Function and Localization

• Perilipin-2, also known as adipophilin or adipose differentiation-related protein (ADRP), is encoded by the Plin2 gene in mouse and is a member of the perilipin family of lipid droplet-associated proteins.
• It functions as a structural component of lipid droplets, essential for the formation and maintenance of intracellular lipid storage droplets, playing a key role in neutral lipid accumulation and metabolism.
• Perilipin-2 is predominantly localized to the membrane of lipid droplets and is a membrane-associated protein.
• It is highly expressed in adipose tissue, particularly in fat pads, indicating its specialized role in adipocyte lipid storage and energy homeostasis.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which may regulate its stability, localization, or function.
• Its molecular weight is approximately 46 kDa (calculated from sequence as 46646 Da), consistent with its detection in Western blot applications.

Experimental Guidance and Technical Tips

• In Western blot, expect a band near 46 kDa; include a positive control such as mouse adipose tissue or cultured adipocyte lysates. Validate signal specificity in your sample system.
• For immunofluorescence, permeabilization may be necessary to detect lipid droplet-associated staining. Consider co-staining with a lipid droplet dye (e.g., BODIPY) for localization confirmation.
• In IHC-P, optimize antigen retrieval conditions and use adipose tissue sections as a positive control. Background can be reduced by adjusting blocking protocols.
• Although validated for ELISA, note that this is a polyclonal antibody; ensure proper capture/detection pairing and standard curve linearity when developing quantitative assays.
• Cross-reactivity with rat has been confirmed, but reactivity in other species should be validated experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Perilipin-2 antibody is part of our comprehensive portfolio for lipid metabolism and metabolic disease targets. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your assay development.