Anti-Perforin Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P14222

Core Product Specifications and Parameters

Biological Background: Perforin-1 Function and Localization

• Perforin-1 (PRF1) is a pore-forming protein crucial for the granzyme-mediated programmed cell death pathway, targeting virus-infected and neoplastic cells. Related references: PMID:20889983 PMID:21037563 PMID:24558045
• Upon calcium binding, perforin inserts into target cell membranes, oligomerizes, and creates large pores, enabling the entry of cytotoxic granzymes that trigger apoptosis. Related references: PMID:20889983 PMID:21037563 PMID:24558045 PMID:20038786 PMID:20225066
• It preferentially delivers cationic proteins, such as granzymes, while neutral or anionic proteins are inefficiently translocated through its pores. Related references: PMID:24558045
• Perforin also plays a key role in immune surveillance, contributing to the killing of non-self cells during transplant rejection and in autoimmune conditions. Related references: PMID:9058810
• Perforin pores mediate the extracellular release of mature caspase-7 (CASP7), further amplifying programmed cell death.
• Subcellularly, perforin is stored in cytolytic granules of cytotoxic T lymphocytes and NK cells, secreted upon target engagement, and can localize to the cell membrane and endosome lumen.
• The protein contains an EGF-like domain, is N-glycosylated, and requires calcium for activity; mutations in PRF1 are linked to familial hemophagocytic lymphohistiocytosis (FHL).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 280-555 of human perforin, encompassing the membrane attack complex/perforin (MACPF) domain, suggesting detection of both full-length and potential cleavage products.
• For western blotting, the predicted molecular weight is approximately 61 kDa; however, post-translational modifications such as glycosylation may alter migration. Validate band size in the relevant sample system.
• Immunohistochemistry and immunofluorescence may require optimization of fixation and permeabilization conditions to expose epitopes in cytolytic granules.
• This antibody shows broad cross-reactivity with mouse and rat, making it suitable for studies across species; however, confirm reactivity in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, connecting diagnostic developers with high-quality reagents like this anti-Perforin polyclonal antibody, suitable for cytotoxicity and immune function research. Beyond single antibodies, CamelBio supports your entire workflow with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing—helping you accelerate development from concept to clinic.