Anti-Perforin Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P14222

Core Product Specifications and Parameters

Biological Background: Perforin Function and Localization

• Perforin-1 (PRF1), also known as cytolysin and lymphocyte pore-forming protein (PFP), is a key effector protein in cytotoxic lymphocytes. Related references: PMID:20889983 PMID:21037563 PMID:24558045
• It plays an essential role in granzyme-mediated programmed cell death, enabling the elimination of virus-infected or neoplastic cells. Related references: PMID:20889983 PMID:21037563 PMID:24558045
• In its calcium-bound form, perforin inserts into the target cell membrane, oligomerizes, and forms large pores that facilitate the passage of cytotoxic granzymes. Related references: PMID:20889983 PMID:21037563
• Perforin promotes cytolysis and apoptosis by mediating the uptake of granzymes into target cells. Related references: PMID:20038786 PMID:20225066 PMID:24558045
• It is important in eliminating cells recognized as non-self, contributing to transplant rejection and some autoimmune diseases. Related references: PMID:9058810
• Deficiencies in PRF1 are associated with familial hemophagocytic lymphohistiocytosis (FHL), a life-threatening immune disorder.
• Subcellularly, perforin is stored in cytolytic granules, secreted upon degranulation, and can associate with the target cell membrane and endosome lumen.
• Post-translational modifications include glycosylation and disulfide bonds; it contains an EGF-like domain and functions as a calcium-dependent, pore-forming transmembrane protein.

Experimental Guidance and Technical Tips

• The immunogen spans the majority of the mature perforin protein (aa 21-555), suggesting broad epitope coverage. Consider verifying specificity via knockout or knockdown models.
• For Western blot, a band around 61 kDa is expected in human and mouse samples. Optimize loading and blocking conditions as needed.
• For IHC-P and IF/ICC, include appropriate permeabilization and antigen retrieval steps; validate in perforin-expressing cytotoxic lymphocytes (e.g., NK cells, CD8+ T cells).
• ELISA suitability indicates potential for quantitative detection; pair with a matched antibody pair for sandwich ELISA development.
• Note cross-reactivity with mouse; validate if species-specific applications require further testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, including validated antibodies like this perforin monoclonal antibody for immunology and cancer research. As a one-stop sourcing bridge, we supply optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support rare-target material acquisition to accelerate your assay development. Inquire about custom antibody development and formulation services tailored to your diagnostic workflows.