Anti-PER3 Rabbit Monoclonal Antibody for WB, ELISA - P56645

Core Product Specifications and Parameters

Biological Background: PER3 Function and Localization

• PER3, also known as period circadian protein homolog 3, GIG13, or FASPS3, is encoded by the PER3 gene (Gene ID 8863) and is a member of the period protein family.
• It is a core component of the circadian clock, a transcription/translation feedback loop (TTFL) that generates approximately 24-hour rhythms in gene expression, regulating metabolism, sleep, body temperature, and other physiological processes.
• PER3 has a redundant role with PER1 and PER2 in circadian rhythm maintenance but is critical for sleep-wake timing and sleep homeostasis, likely through transcriptional regulation of sleep-related genes.
• The protein localizes to both the cytoplasm and nucleus, shuttling between compartments to exert its regulatory functions.
• As part of the negative limb of the TTFL, PER3 interacts with CLOCK/BMAL1 heterodimers to repress its own transcription and that of other clock-controlled genes.
• Post-translational modifications, including phosphorylation and ubiquitin conjugation, regulate PER3 stability and degradation, influencing circadian period length.
• PER3 can bind heme, potentially linking circadian timing to cellular metabolic states.
• Mutations in PER3 are associated with familial advanced sleep phase syndrome (FASPS), highlighting its role in sleep disorders.

Experimental Guidance and Technical Tips

• The immunogen, a recombinant fusion protein covering amino acids 10–100 of human PER3, targets the N-terminal region, which may be conserved across isoforms; verify isoform specificity if required.
• For Western blot, the predicted molecular weight is approximately 132 kDa; use an appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• This antibody is validated for WB and ELISA; consider optimizing dilution and buffer conditions for your specific sample type (e.g., tissue lysates from human, mouse, or rat).
• Cross-reactivity with mouse and rat has been confirmed; validate performance in your model system for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies like this PER3 antibody, and bulk ancillary reagents. Our platform supports diagnostic manufacturers and researchers in circadian rhythm studies by providing reliable access to rare target raw materials and technical consultation from concept to clinic.