Anti-PER2 Polyclonal Antibody for WB, IHC-P, ELISA, ChIP - O15055

Core Product Specifications and Parameters

Biological Background: PER2 Function and Localization

• PER2 is a transcriptional repressor and core component of the circadian clock, generating approximately 24-hour rhythms in gene expression that regulate metabolism, sleep, body temperature, and immune function.
• It functions in the transcription/translation feedback loop (TTFL) by forming a negative limb: together with CRY1/2, it inhibits CLOCK/NPAS2-BMAL1/BMAL2 heterodimer activity, repressing its own expression.
• PER2 also recruits chromatin-modifying complexes (SIN3-HDAC, H3K9 methyltransferases) to target gene promoters and termination sites, regulating transcription initiation and elongation.
• Subcellularly, PER2 localizes to the nucleus, cytoplasm, perinuclear region, and nucleolus, shuttling as part of clock regulation.
• Post-translational modifications including phosphorylation, acetylation, and ubiquitination tune PER2 stability and circadian period length.
• Widely expressed with highest levels in skeletal muscle and pancreas; isoform 2 detected in keratinocytes. Mutations in PER2 cause familial advanced sleep phase syndrome (FASPS).
• PER2 is involved in metabolic regulation, acting as a coactivator of PPARA and corepressor of NR1D1, affecting lipid metabolism and insulin levels.
• It also maintains cardiovascular function by regulating NO and prostaglandin production and controls circadian glutamate uptake in synapses.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 390–565 of human PER2; due to sequence conservation, it may cross-react with mouse and rat PER2 as indicated. Validate cross-reactivity in your target species.
• For western blotting, the predicted molecular weight is ~137 kDa. Use a low‑percentage acrylamide gel and optimize transfer conditions for high‑molecular‑weight proteins.
• For immunohistochemistry (IHC-P), consider testing different antigen retrieval methods and confirm staining specificity in tissues with known expression, such as skeletal muscle or pancreas.
• For chromatin immunoprecipitation (ChIP), include appropriate controls (e.g., knockout cell lines or peptide competition) to verify antibody specificity and target enrichment.
• For ELISA, the antibody may serve as a capture or detection reagent; pairing with a PER2-specific monoclonal antibody could improve assay sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with reliable access to validated antibody raw materials for circadian biology and metabolic disorders. Our supply capabilities include bulk polyclonal antibodies, antigen-specific affinity purification, and complementary reagent sourcing. For PER2 and related clock proteins, CamelBio offers one-stop assistance from lead selection to scale-up, streamlining your path from discovery to clinical application.