Anti-Pepsinogen II (PG II) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P20142

Core Product Specifications and Parameters

Biological Background: Gastricsin (Pepsinogen II/Pepsinogen C) Function and Localization

• Gastricsin, also known as Pepsinogen C or Pepsinogen II, is encoded by the PGC gene in humans.
• It functions as an aspartyl protease and is secreted as a zymogen that hydrolyzes a variety of proteins, playing a key role in protein digestion.
• The protein is secreted and primarily active in the gastric lumen.
• Structural features include disulfide bonds and a signal peptide; it belongs to the peptidase A1 family of aspartyl proteases.
• Alternative splicing generates multiple isoforms of gastricsin.
• The precursor protein has a molecular weight of approximately 42.4 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 73–172; it may detect both the precursor and mature forms of the enzyme. Consider preparing samples that preserve the zymogen or active form as needed.
• For western blotting, the expected band is around 42 kDa. Validate using positive control lysates from stomach tissue or gastric cell lines.
• In immunohistochemistry (paraffin-embedded sections), antigen retrieval may be required. Test on human stomach tissue sections as a positive control.
• For ELISA applications, pair with a suitable detection antibody and optimize conditions using recombinant protein standards.
• Cross-reactivity with mouse and rat is indicated; this antibody may be used in rodent models to study gastric physiology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to high-quality IVD raw materials, including validated antibodies like this anti-Pepsinogen II pAb for digestive enzyme studies. Beyond individual antibodies, we supply optimized antibody pairs, bulk ancillary reagents, and rare-target sourcing to accelerate your assay development from concept to clinic.