Anti-Pepsinogen II (PG II) Rabbit Monoclonal Antibody for IHC-P, ELISA - P20142

Core Product Specifications and Parameters

Biological Background: Gastricsin Function and Localization

• Gastricsin (Pepsinogen C/Pepsinogen II) is an aspartyl protease belonging to the pepsin family, functioning as a digestive enzyme.
• It is secreted as an inactive zymogen (pepsinogen) and activated by autocatalytic cleavage under acidic conditions, indicated by keywords: Zymogen, Aspartyl protease, Hydrolase.
• The protein contains disulfide bonds that stabilize its structure, as noted in the Keywords.
• Subcellular location: Secreted into the gastric lumen, consistent with its role in protein digestion.
• Alternative splicing generates different isoforms, as indicated by the keyword 'Alternative splicing'.
• Molecular weight: 42426 Da (precursor), but the mature enzyme is approximately 42 kDa after signal peptide removal (consistent with the product's 42kDa note).
• Keywords also include 3D-structure, Direct protein sequencing, and Proteomics identification, indicating it is well-characterized.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length precursor (aa 1-388), suggesting the antibody may detect both the zymogen (pepsinogen C) and the mature gastricsin in relevant samples.
• For IHC-P analysis, consider optimizing antigen retrieval conditions, as pepsinogens are secreted proteins and may require mild retrieval.
• In ELISA applications, the recombinant fusion protein used as immunogen could serve as a standard or positive control for quantification.
• Given cross-reactivity with mouse, this antibody may be suitable for translational studies using murine gastric tissue models; however, validation in the target species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

• At CamelBio, we facilitate your IVD development with reliable access to premium antibodies like this Pepsinogen II (PG II) Rabbit mAb.
• Our portfolio covers gastric biomarkers and digestive enzymes, supported by validated antibody pairs, bulk ancillary reagents, and custom raw-material sourcing.
• Partner with us to streamline your journey from concept to clinic.