Anti-Pepsinogen II (PG II) Rabbit Monoclonal Antibody for IHC-P, ELISA - P20142

Core Product Specifications and Parameters

Biological Background: Pepsinogen II (PG II) / Gastricsin Function and Localization

• Gastricsin, also known as pepsinogen C or pepsinogen II, is an aspartyl protease that is secreted as an inactive zymogen.
• Upon activation by acidic conditions and proteolytic cleavage, it converts to gastricsin, which hydrolyzes a broad range of dietary proteins, playing a key role in digestion.
• The protein contains a signal peptide and multiple disulfide bonds, characteristic of secreted proteases, ensuring structural stability in the extracellular environment.
• Alternative splicing of the PGC gene generates multiple isoforms, potentially contributing to functional diversity.
• Its three-dimensional structure has been determined, offering insights into the catalytic mechanism of aspartyl proteases.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant protein; the antibody may recognize both the pro-form (pepsinogen II) and the active enzyme (gastricsin).
• For IHC-P, consider appropriate antigen retrieval methods and validate staining specificity in gastric tissue sections where PG II is abundant.
• In ELISA, validate performance using recombinant protein or native samples; cross-reactivity with mouse and rat orthologs allows testing in preclinical models.
• Because PG II is a secreted protein, sample preparation (e.g., serum, plasma, or tissue supernatant) may influence assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to this anti-Pepsinogen II (PG II) rabbit monoclonal antibody as part of a comprehensive portfolio for gastrointestinal disease research and IVD development. We offer validated antibody pairs, bulk ancillary reagents, and specialized raw materials to support assay development and production. For rare or emerging targets, our sourcing services help secure the critical raw materials you need, from concept to clinic.