Anti-Pepsinogen I (PG I) Rabbit Monoclonal Antibody for ELISA, IHC-P, IF-P - P0DJD7

Core Product Specifications and Parameters

Biological Background: Pepsinogen I (PG I) Function and Localization

• Pepsinogen I, also known as Pepsinogen-4 or Pepsin A-4, is the inactive zymogen precursor of the digestive enzyme pepsin, encoded by the PGA4 gene.
• Upon activation by gastric acid, it is converted into pepsin A, an aspartyl protease with broad substrate specificity; it preferentially cleaves peptide bonds involving phenylalanine and leucine, but also hydrolyzes many other bonds.
• The protein is secreted by gastric chief cells and functions primarily in the stomach lumen, where it initiates protein digestion.
• Pepsinogen I contains a signal peptide and a propeptide that maintain the enzyme in its inactive state until acidic conditions trigger autocatalytic activation.
• The mature pepsin A-4 is a member of the A1 family of peptidases, characterized by two essential aspartic acid residues at the catalytic site.
• According to the UniProt record, it is a phosphoprotein with two conserved disulfide bonds, and its crystal structure has been solved (3D-structure keyword).
• The predicted molecular weight of the preproprotein is approximately 42 kDa (41,977 Da), consistent with the 42 kDa band observed in immunological assays.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to human Pepsinogen I; consider this when designing epitope-specific or competitive assays.
• For immunohistochemistry on paraffin-embedded tissues (IHC-P), optimize antigen retrieval and antibody dilution using known positive control tissues such as gastric mucosa.
• In immunofluorescence (IF-P) applications, ensure proper fixation to preserve antigenicity and reduce background.
• For ELISA, use appropriate coating buffers and validate the detection range with recombinant Pepsinogen I standards; the antibody may be suitable for both indirect and sandwich formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, including validated monoclonal antibodies like this anti-Pepsinogen I clone, bulk ancillary reagents, and sourcing for rare targets. Whether you need ready-to-use antibody pairs or customized procurement solutions for gastric biomarker development, our team connects diagnostic manufacturers and research institutes with reliable supply chains.