Anti-PEPCK/PCK2 Rabbit Monoclonal Antibody (KO Validated) for WB, IF/ICC, ELISA - Q16822

Core Product Specifications and Parameters

Biological Background: PEPCK/PCK2 Function and Localization

• Encoded by the PCK2 gene, also known as PEPCK2 or PEPCK-M, this mitochondrial protein functions as a phosphoenolpyruvate carboxykinase (PEPCK) and a serine/threonine-protein kinase.
• Catalyzes the conversion of oxaloacetate to phosphoenolpyruvate, the rate-limiting step in gluconeogenesis and glyceroneogenesis, using GTP as a phosphate donor (PubMed:28955899). Related references: PMID:28955899
• Its kinase activity phosphorylates and activates ACSL4, thereby promoting ferroptosis, a form of regulated cell death (PubMed:38720107). Related references: PMID:38720107
• Belongs to the PEPCK family of carboxykinases, which play critical roles in energy metabolism and glucose homeostasis.
• Localizes exclusively to the mitochondrion, where it integrates into metabolic pathways of the citric acid cycle.
• Expressed widely across multiple tissues, consistent with its housekeeping metabolic functions.
• Annotated with diverse keywords including Acetylation, Alternative splicing, Decarboxylase, Lyase, Manganese, Metal-binding, Nucleotide-binding, and Transit peptide, indicating complex regulation and enzymatic properties.
• Molecular weight is approximately 71 kDa, but may vary due to post-translational modifications or alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 550-640); the antibody may detect both full-length and truncated forms of PCK2, but validation in relevant sample systems is recommended.
• For Western blot, consider using human, mouse, or rat cell lysates or mitochondrial fractions as positive controls; a band near 71 kDa is expected.
• For immunofluorescence or ICC, mitochondrial staining patterns should be confirmed with organelle-specific markers.
• Due to KO validation, the antibody can be employed in knockout/rescue experiments to demonstrate target specificity.
• ELISA protocols may require immobilization of recombinant PCK2 or capture of the endogenous protein from lysates; perform titration under your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your IVD development. With deep expertise in metabolic targets like PCK2, we can source rare raw materials and provide tailored technical consultation, enabling seamless transition from concept to clinic. Our integrated supply chain ensures reliable access to high-quality reagents for gluconeogenesis, ferroptosis, and related research areas.