Anti-PEPCK/PCK2 Polyclonal Antibody for WB and ELISA - Q16822

Core Product Specifications and Parameters

Biological Background: PEPCK/PCK2 Function and Localization

• PCK2 encodes mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M), a gluconeogenic enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate (PEP) (PubMed:28955899). Related references: PMID:28955899
• Plays an active role in glyceroneogenesis, contributing to triglyceride synthesis from non-carbohydrate precursors (PubMed:28955899). Related references: PMID:28955899
• Additionally functions as a serine/threonine-protein kinase that phosphorylates and activates ACSL4, thereby promoting ferroptosis (PubMed:38720107). Related references: PMID:38720107
• Localizes to the mitochondrion, consistent with its role in linking TCA cycle intermediates to gluconeogenic and glyceroneogenic pathways.
• Widely expressed across human tissues, reflecting its housekeeping role in basal gluconeogenesis and glyceroneogenesis.
• Subject to acetylation and phosphorylation, which may modulate its enzymatic activities and metabolic functions.
• Genetic variants in PCK2 are associated with metabolic disorders, underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-280), which includes the transit peptide and part of the catalytic domain; this region is likely well-conserved across species, aligning with the reported cross-reactivity with mouse and rat.
• For Western blot, expect a band at approximately 71 kDa; note that mitochondrial import may lead to slight variations in observed molecular weight across different cell types.
• ELISA performance can be optimized by titrating the antibody against recombinant PCK2 protein; consider using positive controls such as human liver or kidney lysates where PCK2 expression is known.
• Validate the antibody in relevant sample systems and consider batch-to-batch consistency testing due to the polyclonal nature of the product.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research labs, providing validated antibody pairs, monoclonal/polyclonal antibodies like this anti-PCK2 pAb, and bulk ancillary reagents. Our one-stop platform supports gluconeogenesis and metabolic disorder research with rare target sourcing and technical consultation, accelerating your path from concept to clinic.