Anti-PEN2/PSENEN Rabbit Polyclonal Antibody for WB, ELISA - Q9NZ42

Core Product Specifications and Parameters

Biological Background: PEN2/PSENEN Function and Localization

• PEN2/PSENEN (Presenilin enhancer protein 2) is an essential subunit of the gamma-secretase complex, a transmembrane endoprotease responsible for intramembrane cleavage of integral membrane proteins such as Notch receptors and amyloid-beta precursor protein (APP). This activity is critical for Notch and Wnt signaling cascades. Related references: PMID:12522139, PMID:12679784, PMID:12740439
• The protein is encoded by the PSENEN gene (Gene ID: 55851) and is also known by aliases PEN-2, MDS033, ACNINV2, and MSTP064, highlighting its relevance in multiple pathological contexts.
• As a gamma-secretase auxiliary component, PSENEN modulates presenilin endoproteolysis and overall protease activity, thereby regulating the processing of key regulatory proteins and influencing downstream signaling and cytosolic CTNNB1 levels.
• PSENEN has been shown to play an additional role as a regulator of AMP-activated protein kinase (AMPK) in response to calorie restriction; it binds metformin, facilitating V-ATPase complex inhibition on lysosomes and subsequent AMPK activation via the AXIN1-STK11/LKB1 axis.
• The protein localizes to multiple membrane compartments including the endoplasmic reticulum membrane, Golgi apparatus, Golgi stack membrane, and cell membrane, consistent with its function in transmembrane protein processing.
• It is widely expressed across tissues, with detection in leukocytes, lung, placenta, small intestine, liver, kidney, spleen, thymus, skeletal muscle, heart, and brain, reflecting its fundamental cellular roles.
• Keywords for PSENEN include 3D-structure, Cell membrane, Endoplasmic reticulum, Golgi apparatus, Membrane, Notch signaling pathway, and Transmembrane helix, underscoring its structural and functional characteristics.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide corresponding to amino acids 1-101 of human PEN2/PSENEN (NP_758844.1). This N-terminal region may be suitable for detecting both full-length and processed forms. Researchers should consider the endogenous expression levels of PEN2 in their target samples, as it is ubiquitously expressed but may vary.
• For Western blot (WB), validate the antibody using positive controls such as cell lines or tissues known to express PEN2 (e.g., brain, liver, or leukocyte-derived cells). Optimize blocking and antibody titration to reduce background, given the predicted molecular weight of ~12 kDa.
• In ELISA applications, the synthetic peptide used as immunogen can serve as a positive control for standard curve generation, but assay conditions should be empirically optimized using recombinant protein or cell lysates to ensure specificity.
• Cross-reactivity has been reported for human, mouse, and rat. When analyzing samples from these species, confirm the antibody’s performance with appropriate species-specific controls and consider potential sequence differences in the target region.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio is your strategic partner in IVD raw material procurement, providing one-stop access to high-quality reagents including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-PEN2/PSENEN polyclonal antibody supports research into Alzheimer’s disease, Notch signaling, and gamma-secretase biology, and CamelBio can assist with technical consulting and customized supply solutions to accelerate your diagnostic development from concept to clinic.