Anti-PEN2/PSENEN Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q9NZ42

Core Product Specifications and Parameters

Biological Background: PEN2/PSENEN Function and Localization

• PEN2, also known as presenilin enhancer protein 2, is a 101-amino acid, ~12 kDa transmembrane protein essential for gamma-secretase activity.
• It is a critical subunit of the gamma-secretase complex, which catalyzes intramembrane proteolysis of integral membrane proteins such as Notch receptors and amyloid-beta precursor protein (APP). Related references: PMID:12522139, PMID:12679784, PMID:12740439
• PEN2 modulates presenilin endoproteolysis and is required for the maturation and activity of the gamma-secretase complex.
• The gamma-secretase complex plays a role in Notch and Wnt signaling pathways by regulating the processing of key regulatory proteins and cytosolic CTNNB1 levels.
• Beyond gamma-secretase, PEN2 acts as a regulator of AMP-activated protein kinase (AMPK) activity in response to calorie restriction, binding metformin and promoting V-ATPase complex inhibition.
• Subcellular localization includes the endoplasmic reticulum membrane, Golgi apparatus, Golgi stack membrane, and cell membrane.
• Widely expressed in tissues including leukocytes, lung, placenta, small intestine, liver, kidney, spleen, thymus, skeletal muscle, heart, and brain.

Experimental Guidance and Technical Tips

• For Western blotting, consider using a standard protocol with a 10–15% SDS-PAGE gel to resolve the ~12 kDa PEN2 protein; validate primary antibody dilutions in your specific sample system.
• In immunofluorescence (IF/ICC and IF-P), ensure adequate permeabilization for detection of this membrane protein; optimize fixation and blocking conditions for your cell line or tissue.
• For ELISA applications, test coating antigen and antibody concentrations to achieve optimal signal-to-noise ratios; consider using recombinant PEN2 as a standard.
• Due to high sequence homology, cross-reactivity with mouse and rat is expected; confirm in the relevant species model if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with high-quality IVD raw materials, including validated rabbit monoclonal antibodies like this anti-PEN2/PSENEN clone. For projects focusing on Alzheimer’s disease, Notch signaling, or gamma-secretase complex studies, we offer comprehensive sourcing support—from optimized antibody pairs and ancillary reagents to bulk quantities and hard-to-find targets—streamlining your workflow from concept to clinic.