Anti-PELP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - C9JFV4

Core Product Specifications and Parameters

Biological Background: PELP1 Function and Localization

• PELP1, or Proline-, glutamic acid- and leucine-rich protein 1, is also referred to as Modulator of non-genomic activity of estrogen receptor (MNAR).
• The protein functions in transcriptional regulation, acting as both an activator and a repressor of gene expression according to keyword annotations.
• PELP1 is localized to multiple subcellular compartments, including the cytoplasm, nucleus, nucleolus, and nucleoplasm, reflecting its diverse roles.
• Post-translational modifications such as phosphorylation, acetylation, and ubiquitin-like conjugation have been identified, which may modulate its activity and interactions.
• The protein features repeat sequences (as indicated by the "Repeat" keyword) and is part of the reference proteome for Homo sapiens.
• With a computed molecular weight of approximately 125 kDa (124.9 kDa from the sequence), PELP1 is a relatively large signaling scaffold.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (amino acids 1021–1180) of human PELP1; for specificity verification, consider peptide blocking or knockout/knockdown controls.
• In Western blot (WB), a predominant band near 125 kDa is expected. Optimize transfer conditions for high‑molecular‑weight proteins and validate loading controls.
• For immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), antigen retrieval and appropriate fixation/permeabilization protocols should be established for the target tissue or cell line.
• ELISA performance can be benchmarked using the recombinant immunogen fragment as a positive control. Titration of the antibody and detection reagents is essential for reliable signal‑to‑noise ratios.
• As a polyclonal antibody, lot‑to‑lot variability may occur; include a reference standard in each experiment when quantitative comparisons are needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti‑PELP1 rabbit polyclonal antibody supports research into nuclear receptor signaling, transcriptional regulation, and cancer biology. Our one‑stop sourcing model streamlines procurement for diagnostic manufacturers, ensuring reliable access to critical reagents from concept to clinic.